Identification and Characterization of EST-SSRs in Mungbean

Background: Mungbean has great nutritional sense over other cereal crops and supplements the need of protein and carbohydrates. Mungbean crop improvement programmes through marker assisted selection and gene mapping required molecular markers. In this regards simple sequence repeats (SSR) have great utility mostly because of high polymorphism, co-dominance nature, random distribution and ample availability. Methods: Conventional method for identification of SSRs through enrichment library and sequencing is labour intensive. Available nucleotide sequence data deposited in public domains is one of the major sources for identifying SSRs. Results: In the present study, nucleotide sequences available from NCBI database for mungbean including 829 EST (expressed sequence tags), 83 GSS (genomic survey sequence), and 2903 nucleotide sequences were searched for microsatellite identification and then the characterisation of these SSRs as di, tri and tetra, class I, class II and class III were carried out. The frequency distribution of SSRs concludes that ga/tc (518), ca/tg (238) among di nucleotide and caa/ttg (84) and gaa/ttc among trinucleotide are found most abundantin nucleotide sequence. Conclusions: Moreover EST-SSR markers are also useful to understand population structure and evaluation of genetic diversity which is requisite for the effective utilization of available genetic resources in Vigna species.